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Construction of Hsp90β gene specific silencing plasmid and its transfection efficiency

JI Yewei, NIE Bin, LI Ping, ZHOU Yuanguo, XU Xiaoyu

Frontiers of Medicine 2007, Volume 1, Issue 3, Pages 253-257 doi: 10.1007/s11684-007-0048-9

Abstract: siRNA-like transcripts and thus mediate strong and specific repression of human heat shock protein 90β(Hsp90β) gene expression and to compare the transfection efficiency of the plasmids in varying conditionsThe bases inserted into the plasmids were correct and the positive colonies were named pSuper-Hsp90β1, pSuper-Hsp90β2 and pSuper-Hsp90β3.In conclusion, the si-RNA-synthesizing plasmids targeting Hsp90β were constructed and transfected into

Keywords： HindIII transfection efficiency detection enhanced different

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Four-protein model for predicting prognostic risk of lung cancer

Frontiers of Medicine 2022, Volume 16, Issue 4, Pages 618-626 doi: 10.1007/s11684-021-0867-0

Abstract: Heat-shock protein 90 β (HSP90β) is overexpressed in various tumor cells.In this study, the ELISA results of HSP90β combined with CEA, CA125, and CYFRA21-1 were used to constructResults suggested that the joint detection of HSP90β, CEA, CA125, and CYFRA21-1 in the peripheral blood

Keywords： lung cancer HSP90β decision tree model prognosis

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The Regularity of Time of Arrival in 90° Bend Tunnel for Shock Wave due to Explosions From High Explosive

Shen Jun,Li Yongchi,Pang Weibin

Strategic Study of CAE 2006, Volume 8, Issue 3, Pages 53-57

Abstract:

The propagating regularity and its influencing matter of shock wave in a 90° bend tunnel werecollecting data gained from 4 experiments, a formula was proposed which can compute time of arrival in a 90&This formula can also be used to compute velocity of shock in the 90° bend tunnel.

Keywords： internal explosion shock wave time of arrival

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Prokaryotic expression and purification of fusion protein HSP70-EGFP and its application in the study

QU Ping, SUI Yanfang, MA Jiahai, CHEN Guangsheng, LIU Libing, CHEN Jiankang, LIU Fang'e

Frontiers of Medicine 2008, Volume 2, Issue 1, Pages 82-86 doi: 10.1007/s11684-008-0015-0

Abstract: Fusion protein HSP70-EGFP was prokaryotically expressed, isolated and purified.Group 1 and 2 were respectively incubated for 30 min. with HSP70-EGFP and EGFP.Group 3 was incubated with HSP70 for 30 min, and then incubated for 30 min. with HSP70-EGFP.Fusion protein HSP70-EGFP was successfully obtained and its molecular weight was 97 000.HSP70-EGFP may promote DCs to secret cell factor IL-12.

Keywords： significant difference irradiation peripheral preliminary EGFP